Dxplora diagnostics development and manufacturing coordination
Custom PCR Assays Built for Your Workflow
A custom PCR assay is a target-specific qPCR or RT-qPCR configuration developed around a defined target, sample workflow, instrument, controls, performance goals, and reagent format. Dxplora coordinates development from the inputs already available through evaluation reagents, technical transfer, pilot quantities, and repeat supply planning.
Custom PCR assay development inputs
- Defined target or existing primer-probe set
- Sample matrix and extraction workflow
- PCR instrument, reaction volume, and optical channels
- Controls, performance goals, and preferred reagent format
What a custom PCR assay project can deliver
- Assay architecture and technical development brief
- Optimized singleplex or multiplex reagent configuration
- Evaluation-ready liquid or lyophilized reagents
- Technology transfer, pilot quantities, and repeat supply planning
Flexible project starting points
- Begin with a defined target and build the assay architecture
- Begin with an existing primer-probe set and assess the reaction system
- Begin with a working bench assay and prepare it for transfer and repeat manufacturing
Frequently asked questions
What is a custom PCR assay?
A custom PCR assay is a target-specific qPCR or RT-qPCR configuration developed around a laboratory's defined target, sample workflow, instrument, controls, performance goals, and reagent format rather than supplied as a fixed off-the-shelf test.
What information is needed to develop a custom PCR assay?
Useful starting information includes the target, current primer and probe status, sample matrix and extraction method, instrument and optical channels, reaction volume, controls, available performance data, preferred format, and expected evaluation quantity.
Can a project start from an existing primer and probe set?
Yes, when the existing design is a technical fit. The current sequences, chemistry, controls, instrument, sample matrix, and available performance data are reviewed before a development pathway is proposed.
Can custom PCR assays be supplied as liquid or lyophilized reagents?
Both formats can be assessed. The appropriate presentation depends on chemistry, workflow, storage and shipping goals, reaction volume, packaging, reconstitution requirements, and project-specific feasibility work.